首页> 外国专利> Coating a substrate with a monolayer of self-assembling proteins, comprises providing stabilized aqueous solutions with self-assembling proteins, and contacting a surface of the substrate with the stabilized protein-containing solution

Coating a substrate with a monolayer of self-assembling proteins, comprises providing stabilized aqueous solutions with self-assembling proteins, and contacting a surface of the substrate with the stabilized protein-containing solution

机译:用单层自组装蛋白包被基质,包括为稳定的水溶液提供自组装蛋白,并使基质表面与稳定的含蛋白溶液接触

摘要

The method comprises providing stabilized aqueous solutions with self-assembling proteins, where: the self-assembling protein is provided in an aqueous solution and then an aqueous solution containing a non-ionic surfactant is added in portions to the protein-containing solution from the self-assembling protein in the absence of substances that are suitable for the cleavage of disulfide bonds; and the final concentration of surfactant is calculated according to an equation as given in the specification, and contacting a surface of the substrate with the stabilized protein-containing solution. The method comprises providing stabilized aqueous solutions with self-assembling proteins, where: the self-assembling protein is provided in an aqueous solution and then an aqueous solution containing a non-ionic surfactant is added in portions to the protein-containing solution from the self-assembling protein in the absence of substances that are suitable for the cleavage of disulfide bonds; and the final concentration of surfactant is calculated according to an equation as given in the specification, contacting a surface of the substrate with the stabilized protein-containing solution thus producing a protein-containing coating on the substrate, and removing the supernatant solution from the coated substrate and/or drying the coated substrate. Independent claims are included for: (1) a method for stabilizing solutions with self-assembling proteins; and (2) a substrate with a protein-containing coating.
机译:该方法包括向稳定的水溶液提供自组装蛋白,其中:在水溶液中提供自组装蛋白,然后将包含非离子表面活性剂的水溶液从自身中分部分添加到含蛋白质的溶液中。 -在没有适合二硫键裂解的物质的情况下组装蛋白质;表面活性剂的最终浓度根据说明书中给出的方程式计算,并使底物表面与稳定的含蛋白质溶液接触。该方法包括向稳定的水溶液提供自组装蛋白,其中:在水溶液中提供自组装蛋白,然后将包含非离子表面活性剂的水溶液从自身中分部分添加到含蛋白质的溶液中。 -在没有适合二硫键裂解的物质的情况下组装蛋白质;表面活性剂的最终浓度根据说明书中给出的方程式计算,使基材表面与稳定的含蛋白质溶液接触,从而在基材上产生含蛋白质涂层,并从涂层中去除上清液。基材和/或干燥涂覆的基材。包括以下方面的独立权利要求:(1)用自组装蛋白稳定溶液的方法; (2)具有含蛋白质涂层的基材。

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