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Identifying members of immobilised peptide libraries comprising protein-DNA complexes
Identifying members of immobilised peptide libraries comprising protein-DNA complexes
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机译:鉴定包含蛋白质-DNA复合物的固定化肽库的成员
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摘要
A method for identifying a member from a peptide library that interacts with a target molecule in situ is disclosed, the method comprising providing a plurality of nucleic acid molecules encoding members of the library, immobilising the nucleic acids onto a solid support, sequencing the plurality of nucleic acids on the solid support, expressing the immobilised nucleic acids to produce a peptide library wherein each member of the peptide library is immobilised on the nucleic acid molecule from which it was expressed (e.g. the library is a cis-display library and the peptide binds to a nucleic acid ORI sequence via a RepA or P2A protein sequence), contacting the immobilised peptide library with a target molecule, detecting an interaction between the target and a library member, and identifying the member of the library which interacted with the target by the sequence of the nucleic acid molecule from which it was expressed.
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