Holographic fluctuation microscope apparatus for determining the variance of the motion of and / or cellular particles, method, and program

• 1. 用于确定颗粒和/或细胞分散体的迁移率的全息波动显微装置和方法 [P] . 中国专利： CN103025886A . 2013-04-03
• 2. 用于测量厚度变化的设备、使用该设备的系统、使用该设备的形貌显微镜、测量厚度变化的方法、以及使用该测量方法获取形貌图像的方法 [P] . 中国专利： CN102625902B . 2016.01.06
• 3. HOLOGRAPHIC FLUCTUATION MICROSCOPY APPARATUS AND METHOD FOR DETERMINING MOBILITY OF PARTICLE AND/OR CELL DISPERSIONS [P] . 欧洲知识产权局专利： EP2576805A1 . 2013-04-10

  机译： 全息波动显微镜设备和确定颗粒和/或细胞分散度移动性的方法

• 4. HOLOGRAPHIC FLUCTUATION MICROSCOPY APPARATUS AND METHOD FOR DETERMINING MOBILITY OF PARTICLE AND/OR CELL DISPERSIONS [P] . 美国专利： US2013260396A1 . 2013-10-03

  机译： 全息波动显微镜设备和确定颗粒和/或细胞分散度移动性的方法

• 5. HOLOGRAPHIC FLUCTUATION MICROSCOPY APPARATUS AND METHOD FOR DETERMINING MOBILITY OF PARTICLE AND/OR CELL DISPERSIONS [P] . 日本专利： JP2013531787A . 2013-08-08

  机译： 全息波动显微镜设备和确定颗粒和/或细胞分散度移动性的方法

• 1. The Stepping Motion of Brownian Particle Derived by Nonequilibrium Fluctuation [J] . ZHAN Yong, ZHAO Tong-Jun, YU Hui, 理论物理通讯（英文版） . 2003,第006期
• 2. Global variance reduction method for global Monte Carlo particle transport simulations of CFETR [J] . Xing-Chen Nie, Jia Li, Song-Lin Liu, 核技术（英文版） . 2017,第008期
• 3. A SEM-based method to determine the mineralogical composition and the particle size distribution of suspended sediment [J] . Sylvain Pinet, Bruno Lartiges, Jean-Michel Martinez, 国际泥沙研究（英文版） . 2019,第002期
• 4. Digital holographic microscope for measuring three-dimensional particle distributions and motions [J] . Jian Sheng, Edwin Malkiel, Joseph Katz Applied Optics . 2006,第16期
• 5. Digital holographic microscope for measuring three-dimensional particle distributions and motions [J] . Jian Sheng, Edwin Malkiel, Joseph Katz Applied optics . 2006,第16期
• 6. SYSTEM, PROGRAMMABLE ILLUMINATOR, LITHOGRAPHIC APPARATUS, INCIDENT LIGHT RELATED ENERGY PARAMETER DETERMINING METHOD, AND DEVICE MANUFACTURING METHOD [J] . Research Disclosure . 2021,第689期
• 7. Advancing methods for the analysis of glioblastoma cell motion using quantitative time lapse holographic imaging and cellular tomography [C] . Ed Luther, Livia Mendes, Nina Filipczak, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII . 2019
• 8. Fluctuating immersed material (FIMAT) dynamics for the direct numerical simulation of the Brownian motion of particles. [D] . Chen, Yong. 2005
• 9. Movies of cellular and sub-cellular motion by digital holographic microscopy [O] . Christopher J Mann, Lingfeng Yu, Myung K Kim 2006
• 10. Evaluation of Incipient Motion of Sand Particles by Different Indirect Methods in Erosion Function Apparatus [O] . Arif Jewel, Kazunori Fujisawa, Akira Murakami 2021
• 11. Method and apparatus for determining time, direction, and composition of impacting space particles [R] . 1990

• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. An Alternative Technique for Determining Gravimetric Particle Mass Deposition on Filter Substrate: The Particle Extraction Method [J] . Charity Garland ,Samantha Delapena ,David Pennise . 大气污染开放期刊（英文） . 2018,第4期
• 3. 一种适用于模拟失重环境下长期定量观察生物活细胞的数字全息显微方法 [C] . 潘锋 ,肖文 ,刘烁 . 第一届空间仪器国际学术会议 . 2014
• 4. 双光子荧光显微镜用于在体小鼠下丘脑腹内侧核神经细胞内Ca2+检测的方法学探讨 [A] . 王聪 . 2017