首页> 外国专利> AXMI-115, AXMI-113, AXMI-005, AXMI-163 AND AXMI-184: VIP3A INSECTICIDAL PROTEIN DERIVED FROM BACILLUS THURINGIENSIS AND METHODS FOR USE THEREOF

AXMI-115, AXMI-113, AXMI-005, AXMI-163 AND AXMI-184: VIP3A INSECTICIDAL PROTEIN DERIVED FROM BACILLUS THURINGIENSIS AND METHODS FOR USE THEREOF

机译:AXMI-115,AXMI-113,AXMI-005,AXMI-163和AXMI-184:苏云金芽孢杆菌衍生的VIP3A杀虫蛋白及其使用方法

摘要

PROBLEM TO BE SOLVED: To provide compositions and methods for providing insecticidal activity to a host cell by genetic recombination.SOLUTION: There are provided: a composition comprising a coding sequence encoding delta-endotoxin polypeptide being an insecticidal protein derived from Bacillus thuringiensis; an expression cassette comprising the coding sequence; use of a DNA construct for transformation and expression in a host cell; a host cell transformed by the composition; an isolated nucleic acid molecule encoding delta-endotoxin; an amino acid sequence corresponding to the polynucleotide; an antibody that binds to such an amino acid sequence; an isolated nucleic acid molecule comprising a nucleotide sequence encoding a specified amino acid sequence, a specified nucleotide sequence or a variant or a fragment thereof; and genetic recombinant plant transformed with the composition.
机译:解决的问题:提供通过基因重组为宿主细胞提供杀虫活性的组合物和方法。解决方案:提供一种组合物,其包含编码δ-内毒素多肽的编码序列,所述δ-内毒素多肽是源自苏云金芽孢杆菌的杀虫蛋白;表达盒,其包含编码序列; DNA构建体在宿主细胞中转化和表达的用途;通过该组合物转化的宿主细胞;分离的编码δ-内毒素的核酸分子;对应于多核苷酸的氨基酸序列;与这种氨基酸序列结合的抗体;分离的核酸分子,其包含编码特定氨基酸序列,特定核苷酸序列或其变体或片段的核苷酸序列;以及用该成分转化的基因重组植物。

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