Quantitative nuclease protection assay (qNPA) method and improve quantitative nuclease protection sequencing (qNPS) method

摘要

Quantitative nuclease protection assay (qNPA) method and the present disclosure provides an improved quantitative nuclease protection sequencing (qNPS) method. The disclosed method, using (NPP) nuclease protection probes containing (3-end) flanking sequence to provide amplification and / or sequence universal hybridization arrays, and / or 3 'end 5' end. The disclosed method can be used to detect or sequencing a target nucleic acid molecule, such as nucleic acid molecules present in the sample is insoluble or is fixed.