Its manufacturing method, the measuring device and method for measuring the physiologically active substances of biological origin using the same raw materials and coagulogen

摘要

PROBLEM TO BE SOLVED: To provide a technique for obtaining a coagulogen raw material which can irreversibly inactivate the activity of a coagulase while retaining the function of coagulogen in an LAL reagent, a LAL reagent contaminated by an organism-derived biologically active substance or the like, and which can be used in a reagent.;SOLUTION: An LAL reagent is heated at a predetermined temperature for a predetermined period of time to irreversibly deactivate only the activity of an enzyme contained in the LAL reagent. At this time, such an activity inherent in coagulogen that coagulogen can be hydrolyzed with an activated coagulase and converted to coagulin to induce gelatinization or an agglutination reaction is retained.;COPYRIGHT: (C)2010,JPO&INPIT