Fertilization method using zona methods and media for the preparation of a mammalian egg or embryo is thinning or removal , mammalian eggs prepared by this method .

摘要

Methods of preparing mammalian embryo or egg zona pellucida of the embryo has been removed or thinning, and to provide a fertilization method using mammalian eggs prepared by this method. In a medium containing (eg, DTT or reduced glutathione) a reducing agent having an SH group, by culturing or processing embryo (eg, unfertilized egg, during early embryo or fertilized egg) a mammalian or eggs, and mammals It is a method of removing or thinning of the zona pellucida of the embryo or egg. Embryo (for example, during early embryo unfertilized egg, or fertilized egg) by implantation in vitro fertilization, the fertilized egg, or, of genetically modified animal or mammalian egg zona pellucida, which is provided by such a method has been removed or thinning increase in the incidence and fertilization rate can be achieved by using in the preparation, generation of early embryos to be used in the fabrication.