Pseudonocardia autotrophica expression vector

摘要

This invention regards the production manner of the protein which uses the transformant, and its transformant of shiyudonokarudeia ototorohuika which uses the manifestation vector and that manifestation vector which can reveal the imported gene in shiyudonokarudeia ototorohuika. In addition, it makes the gene reveal which the enzyme which participates in active type vitamin D3 synthesis with transformation internal making use of the above-mentioned manifestation vector and the transformant, the code has been done high-level, it makes the party cutin hydroxide enzyme gene reveal high-level with transformation internal the manner which produces active type vitamin D3 from vitamin D3, furthermore, 25 - the manner which produces hydroxy vitamin D2, making use of the above-mentioned manifestation vector and the transformant from vitamin D2, it regards the manner which produces purabasutachin from party cutin.