Super-resolution optical fluctuation imaging (SOFI)

摘要

Using statistical analysis techniques based on the image stack of the object [Problems] fluctuates, the cross-correlation / cumulants and self, it is possible to improve the resolution beyond the classical diffraction limit, to reduce the background. Time trajectories of all pixels in An image frame is correlated with the time trajectories of adjacent pixels and / or itself. The amplitude of the cross-correlation / cumulants self or each pixel is averaged or time difference of any on interval of the time difference in time difference or integrated is used as the intensity value of the pixels in the super-resolution optical fluctuation generated images. [Selection Figure Figure 2