As isoelectric focusing manner and being the isoelectric focusing manner which

摘要

PROBLEM TO BE SOLVED: To provide an isoelectric electrophoretic method for efficiently removing coarse impurities by the proper setting of a constant voltage process, achieving high throughput and preventing the drying of a gel, and a method for determining the removal of the coarse impurities useful for confirming that the proper constant voltage process is performed.;SOLUTION: The isoelectric electrophoretic method is characterized by performing the constant voltage step of applying a constant voltage of 100-600 V to one specimen-containing gel and starting a voltage raising step of raising voltage from the constant voltage after a current change width per 30 min-migration becomes a 5 μA range.;COPYRIGHT: (C)2011,JPO&INPIT