Isoelectric Focusing of Bacteria: Species Location Within an Isoelectric Focusing Column by Surface Charge.

摘要

The isoelectric point (pI) of each of three cultured bacterial species was determined from collected fractions from an isoelectric focusing column. The pI of fractions containing Streptococcus sanguis 10556 (pH 5.3), Escherichia coli (pH 4.2, 6.4), and Actinomyces viscosus (pH 7.0) differed according to species. Viable recultured bacteria were obtained in 72 hr from the fractions of the pI maxima for each species. Recultured fractions from the pH 4.2 region collected from isoelectric focusing columns which initially contained an equal mixture of S. sanguis, A. Viscosus, and E. coli consisted primarily of S. sanguis. (Author)