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MOLECULAR ASSAY FOR THE AMPLIFICATION AND DETECTION OF KPC GENES RESPONSIBLE FOR HIGH-LEVEL RESISTANCE TO CARBAPENEM IN GRAM NEGATIVE BACTERIA
MOLECULAR ASSAY FOR THE AMPLIFICATION AND DETECTION OF KPC GENES RESPONSIBLE FOR HIGH-LEVEL RESISTANCE TO CARBAPENEM IN GRAM NEGATIVE BACTERIA
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机译:分子检测用于对革兰氏阴性菌中的卡培南具有高水平耐药性的KPC基因进行扩增和检测
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摘要
Methods and kits useful for the detection and identification of carbapenem-resistant pathogens harboring carbapenemase-encoding nucleic acids. Said methods comprise PCR amplification of a target region of the beta-lactamase encoding Klebsiella pneumoniae carbapenemase genes (blaKPC) and variants thereof with a primer set comprising SEQ ID Nos: 1 and 2, and variants thereof
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机译:可用于检测和鉴定带有碳青霉烯酶编码核酸的耐碳青霉菌病原体的方法和试剂盒。所述方法包括用包含SEQ ID Nos:1的引物组对编码肺炎克雷伯菌肺炎克雷伯菌酶(bla KPC Sub>)及其变体的β-内酰胺酶的靶区域进行PCR扩增。和2,及其变体
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