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MOLECULAR ASSAY FOR THE AMPLIFICATION AND DETECTION OF KPC GENES RESPONSIBLE FOR HIGH-LEVEL RESISTANCE TO CARBAPENEM IN GRAM NEGATIVE BACTERIA
MOLECULAR ASSAY FOR THE AMPLIFICATION AND DETECTION OF KPC GENES RESPONSIBLE FOR HIGH-LEVEL RESISTANCE TO CARBAPENEM IN GRAM NEGATIVE BACTERIA
Methods and kits useful for the detection and identification of carbapenem-resistant pathogens harboring carbapenemase-encoding nucleic acids. Said methods comprise PCR amplification of a target region of the beta-lactamase encoding Klebsiella pneumoniae carbapenemase genes (blaKPC) and variants thereof with a primer set comprising SEQ ID Nos: 1 and 2, and variants thereof
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机译:可用于检测和鉴定带有碳青霉烯酶编码核酸的耐碳青霉菌病原体的方法和试剂盒。所述方法包括用包含SEQ ID Nos:1和2的引物组及其变体对编码肺炎克雷伯菌肺炎克雷伯菌酶基因(blaKPC)的β-内酰胺酶的靶区域及其变体进行PCR扩增。
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