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USE OF AT(n) INSERTIONS IN PROMOTER ELEMENTS FOR CONTROLLING THE EXPRESSION LEVELS OF CODING SEQUENCES IN PLANTS

机译:AT(n)插入在启动子元件中用于控制植物编码序列的表达水平

摘要

The present invention refers to the use of AT(n) insertions in promoter elements for controlling the expression levels of coding sequences in plants. The expression levels of the heat shock protein (Gmhsp17.6-L), when compared in resistant and susceptible individuals in the population, demonstrated that the largest expression levels per quantitative PCR were present in the individuals that contained the largest AT insertions in the promoter region. The invention also refers to gene expression cassettes containing promoter regions of the gene with different numbers of AT insertions fused to the GUS protein, for transforming soybean embryos.
机译:本发明涉及在启动子元件中使用AT(n)插入来控制植物中编码序列的表达水平的用途。当在人群中的抗药性和易感人群中进行比较时,热激蛋白(Gmhsp17.6-L)的表达水平表明,在启动子中包含最大AT插入的个体中,每个定量PCR的表达水平最高。地区。本发明还涉及基因表达盒,其包含与GUS蛋白融合的具有不同数目的AT插入的基因的启动子区域,用于转化大豆胚胎。

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