The present invention relates to a novel cyanne derivative having a meso-reaction functional group in a polymethine chain, and a preparation method thereof, and the cyanine derivative having the reaction functional group substituted at the meso site may be suitable for mass production thanks to a very simple synthesis method, have a very fast reaction rate because while a related art reagent for detection of nerve agents undergoes two steps of reactions, the cyanine derivative of the present invention undergoes only one step of reaction, have very excellent sensitivity, and be useful as an acid pH-activated ratiometric NIR probe because it is able to be activated in an acidic pH and is usable in an aqueous environment.
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