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MIR164 gene that controls plant root system development and fertility and use thereof
MIR164 gene that controls plant root system development and fertility and use thereof
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机译:控制植物根系发育和育性的mir164基因及其用途
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摘要
A rice microRNA, miR164 gene, that controls plant root system development and fertility, is obtained through gene isolation, cloning and function verification. Uses of a nucleic acid fragment comprising miR164, which fragment may confer a transformed plant with the ability to increase root number and to alter fertility, wherein the said nucleic acid fragment is selected from one of the following nucleotide sequences: 1) a DNA sequence as shown in SEQ ID NO: 1; 2) a RNA sequence as shown in SEQ ID NO:2; or 3) the conserved sequence of miR164 having the same function as 1) or 2). The nucleotide sequence containing the precursor of miR164 is ligated with an exogenous promoter and introduced into rice to obtain transgenic rice plants which has large root systems but became infertile. The fertility can be restored by external application of phytohormones.
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机译:通过基因分离,克隆和功能验证获得了控制植物根系发育和育性的水稻microRNA miR164基因。包含miR164的核酸片段的用途,该片段可以赋予转化植物增加根数和改变育性的能力,其中所述核酸片段选自以下核苷酸序列之一:1)DNA序列为如SEQ ID NO:1所示; 2)如SEQ ID NO:2所示的RNA序列;或3)具有与1)或2)相同功能的miR164保守序列。将含有miR164前体的核苷酸序列与外源启动子连接,并引入水稻中,以获得具有大根系但变得不育的转基因水稻植物。可以通过外用植物激素来恢复生育能力。
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