Methods for storing human breast milk, preserving its natural flavor and nutritional content. Through the use of an apparatus such as a breast pump, breast milk is expressed from a lactating woman. The milk is transferred through a fill line into a storage container. After the container is substantially filled with breast milk, the fill line is decoupled from the breast pump output, and a vacuum is applied to the line. After the remaining air is evacuated from the container, an isolation valve in the fill line is closed, sealing the container in an evacuated condition. The fill line is removed from the valve, and the container is placed into a freezer or refrigerator for storage. When ready for use, the container is removed from the freezer or refrigerator, the milk is thawed, and the vacuum is broken, allowing removal of the milk for transfer to a bottle.
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