Methods and devices for hybridization and binding assays using thermophoresis

摘要

Methods for performing a hybridization assay between a target biomolecule and an array comprising a surface to which are attached biomolecular probes with different, known sequences, at discrete, known locations, the method comprising: providing a container holding a hybridization mixture comprising the target biomolecule and also holding the array; and creating a temperature gradient in the hybridization mixture oriented within the container such that at least a portion of the target biomolecule is driven onto the surface of the array.