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Cell measuring vessel, extracellular potential measuring method, and chemical testing method

机译:细胞测定容器,细胞外电位测定方法及化学检查方法

摘要

The cell observation using a conventional well plate takes much costs. Each well 3 being opened at a top plate 2a of the well plate 1 has a diameter reduced portion 32 which inner circumferential surface 32a is conically hollowed. At a lower portion of the diameter reduced portion 32 is formed an inserting hole 33. In a detecting portion 4, an outer circumferential surface of a round-bar reference electrode 41 is covered by an insulating portion 42. The detecting portion 4 has an upper end portion 4a and a lower end portion 4b which outer circumferential surface is exposed to an outside without being covered by an insulating portion 42. On an upper side above the lower end portion 4b is formed a diameter expanded portion 4c in which an outer circumferential surface of the insulating portion 42 is covered with a measuring electrode 43. The detecting portion 4 is fixed to a well 3 whose diameter expanded portion 4c is inserted into an inserting hole 33 and its upper end portion 4a is contained in the well 3. In the detecting portion 4, the upper end surface 43a of the measuring electrode 43 is communicated with an inner circumferential surface 32a of the diameter reduced portion 32 positioned in an edge surrounding portion of the inserting hole 33.
机译:使用常规孔板进行细胞观察会花费很多成本。在孔板 1 的顶板 2 a 处开口的每个孔 3 具有直径减小部分< B> 32 的内圆周表面 32 a 呈圆锥形。在直径减小部分 32 的下部形成有插入孔 33 。在检测部 4 中,圆棒状的参比电极 41 的外周面被绝缘部 42 覆盖。检测部 4 具有上端部 4 a 和下端部 4 b < / I>其外周表面暴露于外部而不被绝缘部分 42 覆盖。在下端部 4 b 上方的上侧形成直径扩大部 4 c ,其中绝缘部 42 的外周面被测量电极 43 覆盖。检测部分 4 固定到孔 3 ,其直径扩大部分 4 c 插入到插入孔中 33 及其上端部分 4 a 包含在孔 3 中。在检测部 4 中,测量电极 43 的上端面 43 a 与内部连通。直径减小部分 32 的圆周表面 32 a 位于插入孔 33的边缘围绕部分中。

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