Quality Assessment of induced Pluripotent Stem Cells by reference to Distinctive patterns of splicing of Pluripotency

摘要

Claim 1: Distinctive pattern of splicing that comprises at least one profile and splicing event of Alternative Splicing (as) selected from the group consisting of S15, S9, S18, S19, S23, S26, S30 and S32.Claim 9: Use of the distinctive pattern of splicing as defined in one of the preceding Claims, to determine the condition of Cell Pluripotency.Claim 1: a method for determining the State of Pluripotency cells or groups of cells that will test that comprises the steps of: (i) for each Cell or group of cells.To determine the profile of each Gene splicing and splicing pattern of Distinctive discriminatory; (ii) to calculate the score; and (iii) to compare the discriminatory score with the coordinates of the centroid of a Pluripotent Group and control Group of a differential control; in which Cells or groups of cells to be tested are classified as Pluripotent if your scoreDiscriminatory is closer to the centroid of the Pluripotent control Group centroid Group differential control, and which not otherwise classified as Pluripotent.Claim 17: The Method for determining the State of Pluripotency cells according to the claim that Stage 16, which correlate the distinctive pattern of splicing with the State of Pluripotency of the cells contains either: i) to compare the pattern of Jesus Vo and splicing with a distinctive pattern of splicing of Pluripotent cells.The determination of this distinctive pattern of splicing is similar to, or not statistically different,The distinctive pattern of splicing of Pluripotent cells indicates that these cells are Pluripotent; or (ii) calculate the score to compare the discriminatory and discriminatory score with the coordinates of the centroid of a group control of a Pluripotent and differentiated Group C Ontrol,The cells or groups of cells to be tested are classified as Pluripotent discriminatory if its score is closest to the cluster centroid to the centroid of the Pluripotent Control and differential control in the group are classified as other non Pluripotent Way.Claim 19: a Diagnostic kit comprising means for determining a distinctive pattern of splicing as defined in any of claims 1 to 8 cells.Claim 21: Composition which comprises a Reagent capable of detecting an alternative splicing event or combination of events of Alternative splicing as defined in any of claims 1 to 8 cells.