Process for the microbiological oxidation of aromatic, heterocyclic aromatic, cyclic, straight or branched alkane and alkene compounds using cytochrome p450 monoxygenase bm-3 from bacillus megaterium having a mutation at amino acid 87 or recombinant microorganism expressing the mutant monoxygenase

摘要

The invention relates to novel cytochrome P450 monooxygenases comprising a modified substrate specificity, to nucleotide sequences which code therefor, to expression constructs and vectors containing these sequences, and to microorganisms transformed therewith. The invention also relates to methods for microbiologically oxidizing different organic substrates, such as methods for producing indigo and indirubin.