A PROCESS FOR THE PREPARATION OF AN ECO-FRIENDLY DEHAIRING PROTEASE ENGYME FROM BACILLUS SP. FOR LEATHER PROCESSING

摘要

This invention relates to a process for the production of protease enzyme comprising growing a bacillus sp in a culture medium in a bioreactor, followed by precipitating the cmde enzyme preparation by salt precipitation, subjecting the sarne to centrifugation, to obtain the pelle! followed by dialysis of the pellet against phosphate buffer and lyophitizatiory to obtain the protease enzyme.