PROCESS FOR ISOLATION OF ANTICANCER AGENT A NEW ALKALOID FROM MORINGA OLEIFERA.

摘要

The present invention provides the process for the isolation of new alkaloid from the bark of Moringa oleifera. The process comprises drying, grinding, and hot defatting of M. oleifera bark with petroleum ether. This is followed by the successive sequential hot extraction with the solvents viz; ethyl acetate, acetone, methanol and hydroalcohol. The solvents are then removed under vacuum preferably at a temperature in the range of 35-40 C. The hydroalcoholic crude extract obtained thereby is then subjected to column chromatography in chronological order of polarity of solvent starting with petroleum ether. The acetone fraction showing similar pattern in TLC are mixed together and concentrated in vacuum under reduced pressure to get light brown colour new alkaloid. The crystal is washed to free form any adhere impurities. The crystals are dried and subjected to identification by spectrophotometric methods such as UV, IR, NMR and Mass. The isolated, purified and characterized new alkaloid is subjected to MTT Assay and Capsase-3 activity using cancerous cell line shows potent anticancer activity.