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process for increasing the potential of biofuel production from microalgae using bio modulators

机译:生物调节剂提高微藻生产生物燃料潜力的方法

摘要

the present invention provides a method of treatment of microalgae culture and isolation of extremophiles and marine environments for optimum production of biodiesel. the species of microalgae used may be a species of the genus dunaliella acidophila (d.bardawil, d., d. biolecta, d. lateralis, d. maritima, d. minuta, d. parva, d. d. polymorpha and d. peircei, primolecta.quartolecta pseudosalina, d., d. tertiolecta d. viridis and others). the culture of microalgae for biodiesel production, the optimum conditions for the production of biomass and lipid are different.the invention provides a method to simultaneously stimulate the accumulation of biomass and intracellular lipid (more than 60% of dry weight), mainly intended for the production of neutral lipid by the action of bio diesel, bio natural modulators. the chemical analogues (auxins, gibberellins, cytoquinines.nacl) and alkaline hypersaline conditions. another aspect of the invention is the application of specific changes in ph of the culture medium resulting in the spontaneous precipitation of lipid loaded cells, once the cycle of growth and lipid storage. born in biodiesel production is completed.this method allows the rapid recovery and low energy, passive seeker and the centrifugation step and filtration, microalgale biomass for extraction of intracellular lipids and their conversion to biodiesel.this method is highly selective culture conditions in which only a specified number of species shows a similar response and / or may be, which reduces the risk of contamination by other microalgae, bacteria and fungi, is a problem "column direct in the cropping systems, such as the open pits.this is a system of culture of microalgae and wide application for the biofuel, and others.
机译:本发明提供了一种处理微藻培养物和分离极端微生物和海洋环境以最优化生产生物柴油的方法。所用微藻的种类可以是嗜酸杜氏菌属(d。bardawil,d。,d。biolecta,d.lateralis,d。maritima,d。minuta,d。parva,dd polymorpha和d。peircei,primolecta)。 .quartolecta pseudosalina,d。,d。tertiolecta d。viridis等。用于生产生物柴油的微藻培养物,生产生物质和脂质的最佳条件不同。本发明提供了一种同时刺激生物质和细胞内脂质(干重的60%以上)积累的方法,主要用于通过生物柴油,生物天然调节剂的作用生产中性脂质。化学类似物(生长素,赤霉素,cytoquinines.nacl)和碱性高盐条件。本发明的另一个方面是一旦生长和脂质储存循环,就在培养基的pH值中应用特异性改变,该改变导致脂质负载的细胞自发沉淀。生物柴油生产中诞生的这种方法可以快速回收和低能耗,被动寻找器以及离心步骤和过滤,微藻生物质来提取细胞内脂质并将其转化为生物柴油。此方法是高度选择性的培养条件,其中只有一个指定数量的物种显示出相似的响应,并且可能是这样,这降低了其他微藻,细菌和真菌污染的风险,这是“直接在种植系统(例如露天矿)中出现的问题”。微藻的培养系统和生物燃料等的广泛应用。

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