Method for isolating isomer c9, t11, conjugated linoleic acid

摘要

the subject of the invention is a way of isomers c9, t11 sprzezonego linoleic acid (cla), with the mixture of isomers c9, t11 and t10.c12 cla method enzymatic esterification lipase from candida had cylindracea, charakteryzujacy it, with phase enzymatic esterification cla propan-2-ol in excess molar from 3 to 7.conducted in a temperature from 25 to 35u00b0c and completed in time przereagowania achievers degree level from 15 to 40%.mixture of isomers of methyl esters is preceded by crystallization from solution acetonowego cla at - 75 to - 55u00b0c,the ratio by weight of the methyl esters of cla - objetosciowy to acetone is 3 g: 15 ml to 3 g: 40 mlat the time of at least 10 minutes from achievers by acetone solution crystallization temperature, after which precypitat separates from the supernatant by filtration and then subjected to hydrolysis.