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Method for modifying the natural strains of the Saccharomyces sp. yeast, a modified yeast strain Saccharomyces sp., and a method for isolating resting cells of yeast
Method for modifying the natural strains of the Saccharomyces sp. yeast, a modified yeast strain Saccharomyces sp., and a method for isolating resting cells of yeast
modification of natural strains of the yeast saccharomyces sp., is that natural yeast strain leads to stable haploid through gene deletion ho corresponding conversion types kojarzeniowych and removes the marker troficzny, ura3 gene.so prepared strain yeast modifies to changing at codon found in position 368 gene at codon coding for amn1 on gtt walinu0119 by transformation with plasmids containing homologous sequence previously isolated gene sequence amn1 with position 368 kodonem gtt koduju0105cym wa the rope. the invention also includes a modified yeast strain saccharomyces sp.and the way of isolation cells resting yeast.
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