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Method for modifying the natural strains of the Saccharomyces sp. yeast, a modified yeast strain Saccharomyces sp., and a method for isolating resting cells of yeast

机译:修饰酿酒酵母菌种天然菌株的方法。酵母,改良的酵母菌株Saccharomyces sp。以及分离酵母静止细胞的方法

摘要

modification of natural strains of the yeast saccharomyces sp., is that natural yeast strain leads to stable haploid through gene deletion ho corresponding conversion types kojarzeniowych and removes the marker troficzny, ura3 gene.so prepared strain yeast modifies to changing at codon found in position 368 gene at codon coding for amn1 on gtt walinu0119 by transformation with plasmids containing homologous sequence previously isolated gene sequence amn1 with position 368 kodonem gtt koduju0105cym wa the rope. the invention also includes a modified yeast strain saccharomyces sp.and the way of isolation cells resting yeast.
机译:酵母菌天然菌株的修饰是通过相应的转化类型kojarzeniowych的基因缺失导致天然酵母菌株产生稳定的单倍体,并去除了标记troficzny,ura3基因。因此,制备的酵母菌株会在368位密码子处发生改变通过用含有先前分离的同源序列的质粒的质粒进行转化,该质粒编码在gtt walin u0119上的amn1的密码子,该基因序列位于368 kodonem gtt koduj u0105cym上。本发明还包括修饰的酵母菌株酿酒酵母菌种和分离酵母细胞的方法。

著录项

  • 公开/公告号PL403659A1

    专利类型

  • 公开/公告日2014-10-27

    原文格式PDF

  • 申请/专利权人 UNIWERSYTET JAGIELLOŃSKI;

    申请/专利号PL20130403659

  • 发明设计人 DOMINIKA WŁOCH-SALAMON;

    申请日2013-04-24

  • 分类号C12N1/19;C12N15/01;C12N15/04;C12N15/81;C12R1/85;C12N1/02;

  • 国家 PL

  • 入库时间 2022-08-21 15:56:30

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