METHOD FOR SIMULTANEOUS INDICATION OF MICOPLASM DNA AND VIRAL RNA OF CATTLE STOCK DIARRHEA WITH SUBSEQUENT IDENTIFICATION OF 1 AND 2 GENOTYPE OF CAUSATIVE AGENT OF VIRUS DIARRHEA WITH POLYMERASE CHAIN REACTION

摘要

A method for simultaneous indication of micoplasm DNA and viral RNA of cattle stock diarrhea with subsequent identification of 1 and 2 genotype of causative agent of virus diarrhea with polymerase chain reaction (PCR) includes extraction of nucleic acids, reverse transcription, and amplification. During the first round of PCR the primer systems GPO-1/MGSO and P1/P2 are used, flanking regions of gene 16S rRNA of micoplasms and Eof cattle stock diarrhea virus with forming of fragments having length of 715 and 826 b.p. under the annealing temperature of 55 °C respectively. During the second round of PCR the primer systems TS3/P2 are used for identification of cattle stock virus diarrhea of 1 genotype and TS2/P2 for 2 genotype with synthesis of fragments having the length of 223 and 448 b.p. respectively.