Polypeptides having cellobiohydrolase II activity and polynucleotides for encoding

摘要

Polypeptide having cellobiohydrolase II activity, selected from the group consisting of: br / br / (a) a polypeptide comprising an amino acid sequence, said amino acid sequence has at least 90% identity with the sequence amino acids shown as amino acids 1-477 of SEQ ID NO: 2, br / br / (b) a polypeptide comprising an amino acid sequence, said amino acid sequence has at least 90% identity with the polypeptide encoded by nucleotides 63-1493 of SEQ ID NO: 1, br / br / (c) a polypeptide which is encoded by a nucleotide sequence which hybridizes under very high stringency, br / br / (i) the complementary strand of nucleotides 63 to 1493 of SEQ ID NO: 1, br / br / (ii) the complementary strand of nucleotides 63-563 of SEQ ID NO : 1, or br / br / (iii) the complementary strand of the nucleotides consisting of nucleotides 63-263 of SEQ ID NO: 1, d onde conditions of very high stringency comprise prehybridization and hybridization at 42 in 5X SSPE, 1.0% SDS, 5X Denhardt's solution, 100 microg / ml DNA sheared salmon sperm denatured, and finally washing the carrier three times each material during 15 minutes using 0.1 x SSC, 0.1% SDS at 68C, or, br / br / (d) a fragment of (a) or (b) that has cellobiohydrolase II activity.