Method for obtaining a pure culture of ependymal cells multiciliated.

摘要

The present invention describes a method of obtaining a pure culture of ependymal cells from explants multiciliated ventricular wall adult rodent brain. Ependymal cells are separated from the explant by incubation in a culture medium at low temperature followed by proteolytic digestion. After 24 hours of culture the explant most ependymal cells and 7% of other cell types emerge. Ependymal cells are purified after 48 hours of culture an essential medium without additives or growth factors and low cell density. Under these conditions only survive ependymal cells and few contaminating cells die, so that a pure suspension of ependymal cells is obtained.