diluent for freezing stallion semen, preparation method and applications

摘要

Diluent for freezing stallion semen, preparation method and applications. # The present invention contemplates a means for freezing stallion semen comprising a) a buffer solution of Hank supplemented with HEPES, glucose, lactose, BSA and native phosphocaseinate milk, b ) egg yolk (0.5-4% v / v), c) HCON (CH3) {sub, 2} (1-4% v / v), d) glycerol (0.5-2% v / v ). It also relates to the process for obtaining the means of the invention comprising i) opening an egg and separate the egg yolk, ii) centrifuging the yolk obtained in i) at a speed between 2-2500 rpm for a time between 15-20 minutes, iii) adding the yolk obtained in step ii) 0.5 to 4% v: v solution of Hank's buffer supplemented with HEPES, glucose, lactose, native phosphocaseinate BSA and milk, iv) adding to the solution obtained in iii), HCON (CH3) {sub, 2} 1 to 4% v: v glycerol 0.5 to 2% v / v, and v) homogenizing the solution obtained in iv).