Procedure to remove non-sperm particles in a semen sample obtained from an animal

摘要

Procedure to remove non-sperm particles in a semen sample obtained from an animal in which an analysis of the structure of the sperm chromatin by flow cytometry, wherein said analyzing comprises the acid denaturation of DNA and subsequent staining with acridine orange is performed DNA, comprising: exciting said sample with a laser emitting light of wavelength between 400 and 535 nm, detected green and orange fluorescence emitted from the sample with two photodetectors, using the values ​​of green and orange fluorescence to discriminate particles sperm and sperm not remove non-sperm particles particles analysis. Said animal may be a mammal, for example, sheep, pig, goat, cow, horse and human.