Method for the absolute quantification of peptides by tandem mass spectrometry, and its uses (Machine-translation by Google Translate, not legally binding)

摘要

Method for the absolute quantification of peptides by tandem mass spectrometry, and their uses using isotopically enriched peptides in stable isotopes that present spectral overlap with the peptide of natural isotopic abundance, monitoring of multiple reactions at low resolution in the first analyzer of a spectrometer of tandem masses and multiple linear regression. The method is also applicable for the indirect determination of proteins after the hydrolysis of the proteins with enzymes, the selection of one or several peptides characteristic of these and the absolute determination of these peptides. The invention is applicable in those sectors in which the quantification of proteins and/or peptides in a sample is necessary, such as in biological fluids or in cell cultures, as for example in the sectors of agriculture, chemistry, pharmacy, biomedicine or food safety. (Machine-translation by Google Translate, not legally binding)