Methods for the identification of an isolated strain of a clinical sample at the species and / or subspecies level

摘要

A method for the identification of an isolated strain of a clinical sample, at the species and / or subspecies level, using the MALDI-TOF-MS analysis comprising a stage of classifying the germ in a group before performing the analysis of MALDI-TOF-MS, wherein said MALDI-TOF-MS analysis comprises the step of comparing the spectrum profile of the strain to be identified with a reference database containing the MALDI-TOF- spectra. Characteristic MS of the representative strains of the genospecies of the group of germs to which said strain belongs to be identified, containing said database, for each representative strain, the peaks that have, compared to the peak with the highest intensity arbitrarily set at 1, a relative intensity greater than 0.05, so they are present in at least 2 subcultures