METHOD FOR EFFICIENTLY AMPLIFYING ABNORMAL PRION PROTEIN DERIVED FROM BSE

摘要

A method for efficiently amplifying abnormal prion protein(PrP Sc) derived from bovine spongiform encephalopathy (BSE) isprovided. Ultimately, the invention aims at eradicating thetransmission of a prion disease by detecting a BSE-infected cowearly and developing a method for inactivating prions and permittingearly examination of prion inactivation. Provided is a method forefficiently amplifying PrP Sc derived from BSE, wherein the method isbased on a PMCA (protein misfolding cyclic amplification) method inwhich normal prion protein (PrP C) is used as a source and PrP Sc isused as a seed, and PrP Sc derived from BSE is amplified by stir-mixing,incubating, and sonicating both the PrP C and the PrP Screpeatedly, and wherein the method includes performing stir-mixing-incubation-sonicationin the presence of a polysaccharide sulfate.