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OPTIMIZATION OF EXPRESSION AND PURIFICATION OF RECOMBINANT HUMAN MxA PROTEIN IN E. COLI
OPTIMIZATION OF EXPRESSION AND PURIFICATION OF RECOMBINANT HUMAN MxA PROTEIN IN E. COLI
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机译:重组人MxA蛋白在大肠杆菌中表达和纯化的优化
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摘要
Full length MxA constructs and truncated MxA constructs produce human MxA protein in E. coli. The full length MxA and truncated MxA constructs are preferably E. coli codon-optimized to optimize the amount of protein made using the constructs. T5 or T7 promoters can each be used in combination with either the full length MxA or the truncated MxA constructs. In one preferred embodiment, the MxA protein produced by the full length MxA or truncated MxA constructs is used in a control prep or external control. In other preferred embodiments, the MxA protein is used as a therapeutic.
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