The present invention relates to isolated recombinant proteins, in particular Themis proteins and also to methods of providing isolated recombinant themis protein. Themis (thymocyte-expressed molecule involved in selection),is a member of a family of proteins whose functions are not all known. It is highly conserved among vertebrates. Themis has been found to have high expression in thymocytes between the pre-T cell antigen receptor (pre-TCR) and positive-selection checkpoints and low expression in mature T cells. Themis protein is not easy to produce and purify in large quantities. The present invention provides an isolated, soluble protein comprising an amino acid sequence selected from: a) the sequence set out in SEQ ID NO: 1; b) the sequence of amino acids 1 to 261 of SEQ ID NO: 1; c) a sequence having at least 70% identity to a) or b); d) an active fragment or variant of a), b) or c); e) a fusion protein comprising the sequence of a), b), c) or d) and a fusion partner; f) a sequence comprising the sequence of a), b), c), d) or e) and a purification tag.
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机译：本发明涉及分离的重组蛋白质，特别是Themis蛋白质，还涉及提供分离的重组themis蛋白质的方法。 Themis（参与选择的表达胸腺细胞的分子）是功能未知的蛋白质家族的成员。在脊椎动物中它是高度保守的。已经发现，mis虫在胸腺细胞中在前T细胞抗原受体（pre-TCR）和阳性选择检查点之间高表达，而在成熟T细胞中低表达。 Themis蛋白不易于大量生产和纯化。本发明提供了分离的可溶性蛋白质，其包含选自以下的氨基酸序列：a）SEQ ID NO：1所示的序列;和b）SEQ ID NO：1的氨基酸1至261的序列; c）与a）或b）具有至少70％同一性的序列; d）a），b）或c）的活性片段或变体; e）包含a），b），c）或d）的序列和融合伴侣的融合蛋白; f）包含a），b），c），d）或e）的序列和纯化标签的序列。
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