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THERMOSTABLE ENZYME-BASED EXTRACTIONS ON AN INTEGRATED MICROFLUIDIC CHIP FOR BIOLOGICAL ANALYSIS
THERMOSTABLE ENZYME-BASED EXTRACTIONS ON AN INTEGRATED MICROFLUIDIC CHIP FOR BIOLOGICAL ANALYSIS
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机译:用于生物分析的集成微流片上基于热酶的提取物
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摘要
liquid extraction methods are used to recover nucleic acid samples in a fully-automated microfiuidic chip tor biological analysis. A sample is introduced to a rnicrofiuidic chip through a sample acceptor that can be isolated from the surroundings, but. fluidly coupled to a first domain containing a pre-loaded liquid extraction solution. The liquid extraction solution uses one or more thermostable proteinases to extract DNA from the biological sample by submerging an introduced sample and returning with an extracted DNA solution to the first domain, A fraction of the resultant extracted DNA solution is directed through a side channel toward the second domain, where it mixes with PGR reagents and a fraction of pre-PCR solution is thermally cycled in a PGR reaction chamber. The amplified DMA solution is then mixed with a separations solution for separation by capillary electrophoresis and detection by a DNA Analyzer, After the first extraction step, the sample solution is diluted at every processing step.
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