A COMBINED SCALABLE IN VITRO DIFFERENTIATION SYSTEM FOR HUMAN EMBRYONIC STEM CELLS FOR DIRECT ASSAY APPLICATION IN MULTIWELL PLATES

摘要

The present invention relates to a combined scalable in vitro differentiation and assay system based on human blastocyst-derived stem (hBS) cells or cells derived from hBS cells. The present invention makes it possible to merge both the differentiation and the assay parts of the system into one. The advantage of the combined assay system is that the hBS cells or the cells derived from hBS cells in the differentiation system are directly applicable for assays, in large variety of assay units, such as different multiwell plates. The herein presented system is therefore a major improvement compared to the prior art, since the cells are differentiated in the same format as they are further being subject to analysis in. The starting cell material can be homogenously distributed across a variety of different plates, for use and can be cultured attached, semi attached or in suspension.