IMPROVED HIGH THROUGHPUT SYSTEM FOR GENETIC STUDIES

摘要

Improvements in methods to obtain and characterize the genome of tetrad spores by providing fluorescent markers and/or barcodes are disclosed. The invention relates to improvements in techniques for studying the effects of genetic mutations on phenotypes and interactions between genes and environmental factors. A classic method for such study based on sporulation of tetrads in yeast and other microorganisms that undergo similar behaviors is adapted for high throughput analysis by providing mioticbased fluorescent labeling and the use of barcodes.