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A SIMPLE AND AFFORDABLE METHOD FOR IMMUOPHENOTYPING USING A MICROFLUIDIC CHIP SAMPLE PREPARATION WITH IMAGE CYTOMETRY
A SIMPLE AND AFFORDABLE METHOD FOR IMMUOPHENOTYPING USING A MICROFLUIDIC CHIP SAMPLE PREPARATION WITH IMAGE CYTOMETRY
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机译:使用图像细胞仪进行微流片芯片样品制备的一种简单而经济的免疫分型方法
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摘要
The system includes a simple system for CD4 and CD8 counting in point-of-care HIV staging within resource poor countries. Unlike previous approaches, no sample preparation is required with the sample added directly to a chip containing dried reagents by capillary flow. A large area image cytometer consisting of an LED module is used to excite the fluorochromes PerCP and APC labeled targets and a monochrome CCD camera with a combination of two macro lenses captures images of 40 mm2 of blood (approximately 1 micro liter). CD4 and CD8-T-lymphocyte counts correlate well with those obtained by flow cytometry. The cytometer system described in the present invention provides an affordable and easy-to-use technique for use in remote locations.
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