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Simple and affordable method for immuophenotyping using a microfluidic chip sample preparation with image cytometry
Simple and affordable method for immuophenotyping using a microfluidic chip sample preparation with image cytometry
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机译:使用图像流式细胞术的微流控芯片样品制备方法进行免疫分型的简单且负担得起的方法
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摘要
The system includes a simple system for CD4 and CD8 counting in point-of-care HIV staging within resource poor countries. Unlike previous approaches, no sample preparation is required with the sample added directly to a chip containing dried reagents by capillary flow. A large area image cytometer consisting of an LED module is used to excite the fluorochromes PerCP and APC labeled targets and a monochrome CCD camera with a combination of two macro lenses captures images of 40 mm2 of blood (approximately 1 microliter). CD4 and CD8-T-lymphocyte counts correlate well with those obtained by flow cytometry. The cytometer system described in the present invention provides an affordable and easy-to-use technique for use in remote locations.
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机译:该系统包括一个简单的CD4和CD8系统,用于在资源匮乏的国家/地区对即时医疗艾滋病分期进行计数。与以前的方法不同,不需要通过直接将样品通过毛细管流添加到包含干燥试剂的芯片中的样品制备方法。由LED模块组成的大面积图像流式细胞仪用于激发荧光染料PerCP和APC标记的靶标,并且带有两个微距镜头的单色CCD摄像头可捕获40 mm 2 Sup>的血液图像(大约1微升)。 CD4和CD8-T淋巴细胞计数与通过流式细胞仪获得的计数非常相关。本发明中描述的细胞仪系统提供了一种在偏远地区使用的负担得起且易于使用的技术。
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