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CELL WALL DECONSTRUCTION ENZYMES OF MALBRANCHEA CINNAMOMEA AND USES THEREOF

机译:肉桂的细胞壁解构酶及其用途

摘要

The present invention relates to a novel process for degrading biomass or pretreated biomass to sugars wherein an enzyme is used comprising: a. a polypeptide comprising an amino acid sequence of any one of SEQ ID NOs: 5 or 6; b. a polypeptide comprising an amino acid sequence that is at least 70%, preferably at least 80%, more preferably at least 90%, even more preferably at least 95%, 96%, 97%, 98% or 99% amino acid sequence identity to the polypeptide defined in (a); c. a polypeptide comprising an amino acid sequence encoded by the nucleic acid sequence of any one of SEQ ID NOs: 1-4; d. a polypeptide comprising an amino acid sequence encoded by a polynucleotide molecule that hybridizes under medium-high stringency conditions, high stringency conditions, or very high stringency conditions with the full-length complement of a polynucleotide molecule comprising the nucleic acid sequence of any one of SEQ ID NOs: 1-4; e. a polypeptide comprising an amino acid sequence encoded by a polynucleotide molecule having at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% nucleic acid sequence identity to a polynucleotide comprising the nucleic acid sequence of any one of SEQ ID NOs: 1-4; or f. a polypeptide comprising an amino acid sequence encoded by any one of the exonic nucleic acid sequences corresponding to positions: 1-194, 276-568, 639-809, 869-1143, 1235-1322, 1386-1797, 1872-2040of SEQ ID NO: 1; 1-729 of SEQ ID NO: 2.
机译:本发明涉及一种用于将生物质或预处理的生物质降解为糖的新方法,其中使用的酶包括:a。多肽,其包含SEQ ID NO:5或6中任一项的氨基酸序列; b。包含至少70%,优选至少80%,更优选至少90%,甚至更优选至少95%,96%,97%,98%或99%氨基酸序列同一性的氨基酸序列的多肽(a)中定义的多肽; C。多肽,其包含由SEQ ID NO:1-4中任一项的核酸序列编码的氨基酸序列; d。一种多肽,其包含由多核苷酸分子编码的氨基酸序列,所述多核苷酸分子在中等高严格条件,高严格条件或非常高严格条件下与包含SEQ ID NO:1的任意核酸序列的多核苷酸分子的全长互补序列杂交ID编号:1-4; e。包含由多核苷酸分子编码的氨基酸序列的多肽,所述多核苷酸分子具有至少70%,至少75%,至少80%,至少85%,至少90%,至少91%,至少92%,至少与包含SEQ ID NO:1中任一项的核酸序列的多核苷酸的核酸序列同一性为93%,至少94%,至少95%,至少96%,至少97%,至少98%或至少99%。 ID编号:1-4;或f。多肽,其包含由与位置ID的1-194、276-568、639-809、869-1143、1235-1322、1386-1797、1872-2040相对应的任何外显子核酸序列编码的氨基酸序列否:1; SEQ ID NO:2的1-729。

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