Method of calibrating mass-to-charge ratio measurements obtained from a mass spectrometer connected in fluid communication with an analysis system delivering a temporally changing sample

摘要

A method of calibrating mass-to-charge ratio measurements obtained from a mass spectrometer connected in fluid communication with a chromatographic system comprises the steps of acquiring a multiplicity of mass spectra of an analyte during an analytical run, analyzing at least some of the multiplicity of mass spectra during the analytical run for generating result data relating to a decision on introduction of a calibrant material into the mass spectrometer; and introducing the calibrant material into the mass spectrometer during the analytical run, based on the result data. An arrangement for mass analysis that is suitable for carrying out the method comprises a chromatographic system (10), a mass spectrometer (20) connected downstream, in fluid communication to the chromatographic system (10), a data acquisition device (22) for acquiring mass spectra of an analyte fed to the mass spectrometer (20), a controllable injector (30, 31) to introduce calibrant material into the mass spectrometer, and a control unit (50) receiving information on the acquired mass spectra from the data acquisition device (22), the control unit (50) configured to dynamically control the injector (30, 31) based on the received information.