Method of a test strip detecting concentration of an analyte of a sample and test strip with three electrodes

摘要

A method of a test strip (100, 600, 1100) detecting concentration of an analyte of a sample includes placing the sample in a reaction region (302, 602) of the test strip (100, 600, 1100), wherein the analyte reacts with an enzyme to generate a plurality of electrons, and the plurality of electrons are transferred to a working electrode of the reaction region (302, 602) through a mediator; applying an electrical signal to the working electrode; measuring a first current through the working electrode during a first period; the mediator generating during a second period an intermediate according to the electrical signal; measuring during a third period a second current through the working electrode; calculating initial concentration of the analyte according to the first current; calculating a diffusion factor of the intermediate in the sample according to the second current; and correcting the initial concentration to generate new concentration of the analyte according to the diffusion factor.