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METHOD FOR SOLUBLE OVEREXPRESSION AND PURIFICATION OF RECOMBINANT HUMAN BMP 7 IN ESCHERICHIA COLI
METHOD FOR SOLUBLE OVEREXPRESSION AND PURIFICATION OF RECOMBINANT HUMAN BMP 7 IN ESCHERICHIA COLI
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机译:可溶性人类BMP 7在大肠杆菌中的可溶性过表达和纯化方法
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摘要
The present invention relates to a method for soluble overexpression and purification of recombinant human bone morphogenetic protein (BMP 7) in Escherichia coli, and provides a recombinant expression vector including a solubility enhancing tag and a human BMP 7 gene. More specifically, the solubility enhancing tag is human protein disulfide isomerase (hPDI), glutathione-S-transferase (GST), or maltose binding protein (MBP). In addition, the present invention provides a transformed recombinant microorganism as the recombinant expression vector. Moreover, the present invention provides a method for producing recombinant human BMP 7 (rhBMP7) and recombinant human BMP 7 (rhBMP7) produced by the method.;COPYRIGHT KIPO 2014
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