Method for Preparing Transformed Escherichia coli for Over-expression of Fatty Acid

摘要

invention for fatty acid over-expression of the transformed E. coli according to the manufacturing method and, more particularly, ( a) fabB (3- oxo-acyl-[acyl-transfer-protein] synthase ), fabG (3- oxo-acyl-[acyl-transfer-protein] Lee reductase kinase), fabZ (3R- pre-hydroxy acyl transfer protein dehydration Alpharetta Ajay stall) or fabI (Eno day - [acyl-transfer-protein] reductase kinase ) for inserting at least one nucleotide sequence selected from the group consisting of a nucleotide sequence coding for the expression vector phase; And (b) of the fabB , fabG , fabZ and fabI is inserted into an expression vector encoding a nucleotide relates to a step of transforming E. coli. According to the present invention, the present invention can provide a method for producing a transformed E. coli for the expression of fatty acid biosynthesis pathway, the glucose provides a recombinant E. coli of the fatty acid elongation step of converting a hydrocarbon. The present invention also can increase the production of fatty acid biosynthesis metabolites of the microorganism according to one or more genes in combination. ;