Nucleus mimic, gene-networked gel matrix for in vitro protein production, and preparation method thereof

摘要

The present invention provides an expression plasmid, transcription and translation constituents, including the X-type nucleic acid nanostructures, DNA fragments , comprising a lipid membrane components, cell nucleus simulation relates to cell-free nucleic acid gel matrix and a method for producing a protein-synthesis. Nucleic acid gel matrix for cell-free protein synthesis according to the present invention is X-type nucleic acid nanostructures, the expression plasmid containing the DNA fragment, transcription and translation constituent material, and containing the polymorphism of the lipid membrane components (eg, oval, rectangular or square as the gel matrix), cell nuclei in the interior of the small space due to the model simulated the physical structure (oval) of the chromosomal DNA, transcription and translation factors and the like are concentrated here to be configured to maximize protein production, as well as the gel form contains the DNA inside it for a long period of time because they can be protected from external factors may be used to stably produce high concentrations of protein. ;