hydroxy steroid dehydrogenase using , oxo steroid or steroid -hydroxy compound by reduction of compound process for the preparation of a steroid derivative according to the oxidation

摘要

includes one or more heteroatoms in the ring structure, one or more double bonds and / or aromatic have a steroid structure (ABCD) that, in the steroid ring system 3, 7, 11, 12 or 17 to the position or the steroid skeleton (= oxo-steroid compound / hydroxy steroid compound) at least one radical in the - position of any carbon on the A process for the selective nature enantiomer (enantioselective) enzymatic reduction of an oxo compound with, ;, where the oxo steroid compounds may be reduced by using the hydroxy-steroid dehydrogenase in the presence of NADH or NADPH cofactor (cofactor), ; a) the oxo-steroid compound is present in a concentration of 50 g / l in the reaction mixture, ; b) formed by the hydroxy-steroid dehydrogenase, an oxidized cofactor NAD or NADP is represented by the general formula R _X R _Y by oxidation of the secondary alcohol of the CHOH or C4-C6- and reproduced continuously by oxidation of a cycloalkane ol (where R _X , R _Y are each hydrogen, branched (branched) or linear (unbranched) C1-C8- alkyl and C _total independently represents a 3), and ; c) additional redox enzyme (oxidoreductase) / alcohol dehydrogenase of the general formula R _X R _Y is used for the oxidation of secondary alcohols or cycloalkane all of CHOH ; is characterized in that.