HUMAN ERYTHROID PROGENITOR CELL LINE AND METHOD FOR PRODUCING HUMAN ENUCLEATED RED BLOOD CELLS

摘要

The present invention provides a method for producing an immortalized human erythroid progenitor cell line and a method for producing enucleated human red blood cells from the human erythroid progenitor cell line obtained by the method in order to produce the enucleated human red blood cells efficiently and stably. To solve the project, an expressing cassette capable of inducing HPV-E6/E7 gene expression into the genomic DNA of blood stem cells under the presence of DOX was introduced. And, the immortalized human erythroid progenitor cells were established by culturing the blood stem cells under the presence of the DOX and hematopoietic growth factors. Also, the result that blood stem cells can be differentiated into the enucleated red blood cells if culturing under the absence of HPV-E6/E7 gene expression was found out. [Reference numerals] (AA) Cell number; (BB) Days of culture