METHOD OF SEPARATING AND PURIFYING ISOFLAVONE AND SOYASAPONIN FROM SOYBEAN EMBRYOS

摘要

The present invention relates to a method of separating and purifying isoflavone and soyasaponin from soybean embryos, and more specifically, to the method of separating and purifying isoflavone and soyasaponin from soybean embryos in which a processing time is reduced by separating extracts containing embryos separated from soybeans through preparative chromatography, thereby separating the isoflavone, which is a bioactive substance, and saponin at the same time; has excellent workability; is capable of purifying the isoflavone and saponin having high chemical purity; and can recycle soybean byproducts that are treated as waste, thereby reducing environmental pollution.;COPYRIGHT KIPO 2014;[Reference numerals] (AA) Dry raw beans (40°C, 72 hours); (BB) Separate soybean embryos (sifter with 2.36, 2.0, 1.18 mm); (CC) Remove fat of hexane (room temperature, 48 hours); (DD) Extract methanol (90°C, reflux cooling extraction 6 hours, twice); (EE) Condense and remove protein and sugar (twice); (FF) Separate by a C_18 (125?, 55-105쨉m) column catridge; (GG) Divide isoflavone (ISF-1); (HH,LL) Freeze-dry; (II) Re-separate ISF-1 (gel permeation column); (JJ) Check characteristics of isoflavone (ISF-1-1. ISF-1-2); (KK) Divide four kinds of soyasaponin; (MM) Check a molecular weight of the soyasaponin (Q-TOF) (SP-1,SP-2,SP-3,SP-4)